Purification of Histidine‐tagged and GST tagged proteins using ÄKTA™ start (LB264)
نویسندگان
چکیده
منابع مشابه
Challenges and opportunities in the purification of recombinant tagged proteins.
The purification of recombinant proteins by affinity chromatography is one of the most efficient strategies due to the high recovery yields and purity achieved. However, this is dependent on the availability of specific affinity adsorbents for each particular target protein. The diversity of proteins to be purified augments the complexity and number of specific affinity adsorbents needed, and t...
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متن کاملExpression, purification, interactions of proteins and helicase assays GST-tagged or His-tagged proteins were expressed in bacterial cells according to standard protocols at 18oC and subsequently purified by binding to either Glutathione S-Sepharose (GE
hTERT-immortalized BS fibroblasts GM03509 (referred as BS) and chromosome 15 minichromosome-corrected BS fibroblasts (referred as A-15) (donated by Jerry Shay), GFP-BLM expressing BS fibroblasts GM08505 (referred as GFP-BLM) (donated by Nathan Ellis) were maintained with DMEM supplemented with 10% FCS, glutamine, sodium pyruvate and antibiotics. hTERT-immortalised RIDDLE patient cell line expre...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2014
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.28.1_supplement.lb264